Isolation and identification of Biofilm-producing E. coli from drinking water.

Background: The increasing rate of water-borne diseases in Karachi demands the characterization associated pathogens. Nowadays, infections become more resistant to treatment due biofilm formation and excessive use antibiotics. Biofilm water deteriorates quality water. This study identified characterized biofilm-producing E. coli drinking Methodology: Water samples were analyzed for heterotrophic plate count total coliforms, fecal coli. Kirby disk bar diffusion method was used investigate antibiotic susceptibility Congo red tube ring methods identify producers. effect on hydrophobicity performed by BATH method. soft agar determines colony spreading ability non-biofilm Molecular virulence genes a PCR. Scanning electron microscopy construction conducted. Results: 120 tested 78% unfit this study, 21.66% fit. 38 strains found samples. According findings, isolates increased with incubation period. Colony-forming unit drops one logarithm state compared planktonic stage. producers genes, pet, lt, stx2, molecular characterization. Conclusion: presence is alarming, it indicates inappropriate supply system. To prevent rapid diseases, adequate actions are required control drinking-water